Development of bovine embryos in vitro following oocyte maturation under defined conditions

نویسندگان
چکیده

منابع مشابه

Development of bovine embryos in vitro following oocyte maturation under defined conditions.

A total of 4,615 immature bovine oocytes were used in a series of experiments aimed at the systematic evaluation of the role of the different components of our in vitro maturation (IVM) medium in imparting developmental competence to the oocytes. The results clearly demonstrate that both tissue culture medium 199 (M199) and synthetic oviduct fluid (SOF) are capable of supporting the IVM of bovi...

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Embryological Aspects of Oocyte In Vitro Maturation

In Vitro Maturation (IVM) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. This review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. The review is based on literature reports and the author’s experience. In IVM cycles,...

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Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

The aim of this study was to identify the in vitro development stage at which the culture of a single or low number (n = 5 or 10) of oocytes/embryos could impair development in comparison with culture in group (n = 50). In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group (49.4 vs. 83.0%; 0% vs. 37.8%, respectivel...

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Melatonin Impact on In Vitro Development of Mouse Preantral Follicles and Oocyte Maturation

Introduction: Melatonin acts as an indirect antioxidant and is a powerful direct free radical scavenger and direct responses to melatonin in the gonads are detected. This study aims to investigate the influence of different doses of melatonin on preantral follicle development and oogenesis of in vitro cultured mouse ovarian follicles. Materials and Methods: Preantral follicles with diamet...

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In vitro production of bovine embryos: revisiting oocyte development and application of systems biology

In vitro production (IVP) of bovine embryos has become a widespread technology implemented in cattle breeding and production. The implementation of genomic selection and systems biology adds great dimensions to the impact of bovine IVP. The physical procedures included in the IVP process can still be improved, and aspects related to the oocyte donor, oocyte maturation and the recipients are add...

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ژورنال

عنوان ژورنال: Reproduction Nutrition Development

سال: 1994

ISSN: 0926-5287

DOI: 10.1051/rnd:19940405